Research Paper on Gel Electrophoresis Essay Example

Research Paper on Gel Electrophoresis Essay Gel Electrophoresis is a technique in molecular biology and biochemistry, which is used for the separation of proteins and nucleic acids, based on the motion of charged biological macromolecules in a constant electric field. Polyacrylamide gel separation is due to differences in molecular charge and separated differences of molecular weights and the configuration of molecules. There are so-called non-denaturating or native PAGE electrophoresis (where the shared biological macromolecules are in the native state during electrophoresis) and denaturing polyacrylamide gel electrophoresis (where the samples were denatured beforehand in the case of nucleic acids using short heating the sample with glyoxal or formamide, to denature the proteins normally boiling the samples used in buffer containing a strong ionic detergent (usually sodium dodecyl sulphate) and the agent that disrupts the quaternary structure of the protein by breaking disulfide bridges between protein globules inside polypep tide chain – beta-mercaptoethanol). During the process of denaturing by PAGE electrophoresis, molecules are stored in the denatured state by the presence of chaotropic agents in the gel (typically urea) in the case of the PAGE electrophoresis of nucleic acids and proteins through the presence of the ion (e.g. sodium dodecyl sulfate, cetyltrimethylammonium bromide) and non-ionic (e.g., tween-20) detergents. In the case of polyacrylamide gel electrophoresis of proteins method the modification of Laemmli is generally used. Also polyacrylamide gel electrophoresis is used to separate the short nucleic acid fragments, e.g., DNA electrophoresis, in cases such as Sanger sequencing. Additionally, SDS-PAGE electrophoresis is used in methods for visualizing the restriction fragment length polymorphismand polymerase chain reaction method. We will write a custom essay sample on Research Paper on Gel Electrophoresis specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Research Paper on Gel Electrophoresis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Research Paper on Gel Electrophoresis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer There is also so-called disc-electrophoresis (from discontinuous), where there is pH gradient in the gel during the electrophoretic separation of proteins at the interface between the concentrating and separating gels, thereby achieving better separation of protein molecules. When you are about to write your first research proposal, it is very important to carefully study all the various aspects of the investigating phenomena. You will have to thoroughly investigate the origin, history, and contribution of the subject in modern science. In addition, your own ideas on the methods and techniques of the process improving and development would be appreciated. To prepare a successful research paper on gel electrophoresis, you will need to find the best way to present the result of your investigation on paper. If you have no or small experience, it might be very helpful to look through some free sample research papers on different topics. These free example topics will teach you how to properly prepare and structure your data, how to outline your thesis statement and emphasize the most important passages of your text. In addition to this, you will learn how to clearly and persuasively conclude your research paper. At EssayLib.com writing service you can order a custom research paper on Gel Electrophoresis topics. Your research paper will be written from scratch. We hire top-rated Ph.D. and Master’s writers only to provide students with professional research paper assistance at affordable rates. Each customer will get a non-plagiarized paper with timely delivery. Just visit our website and fill in the order form with all research paper details: Enjoy our professional research paper writing service!